This cluster of papers focuses on the epidemiology, treatment, and prevention of Clostridium difficile infection, with a particular emphasis on fecal microbiota transplantation, antibiotic-associated diarrhea, healthcare-associated infections, toxin production, and clinical practice guidelines. It also explores the role of microbiome diversity, vancomycin and metronidazole treatment, risk factors for infection, and bacterial toxins associated with the disease.